Virtual microscope lab

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Source Lasers Hg or Xenon arc lamp Detector CCD, EMCCD PMT Line CCD CCD, EMCCD Microscope Flexible Specific Flexible / Specific Flexible Scan speed of full-size image 2000fps ~1fps ~120fps (512X512) Any Photo bleaching/ photo toxicity Low Severe Low Confocality High (X-Y-Z confocal) Modest (Compromise y-resolution) Modest (X-Y-Z confocal with pinhole, Compromise y-resolution with slit-scan) None Image Quality (Background) High Good S/N (Low background) High (multiple averaging necessary) Modest Modest (High background with dim samples) Low (High background) User labs Ted Salmon Lab., Dept. of Biology, University of North Carolina, Chapel Hill Waterman-Storer Lab., Laboratory of Cell and Tissue Morphodynamics (LCTM),NHLBI（Bethesda Campus) Tim Mitchison Lab., Dept. of Systems Biology, Harvard Medical School Scholey Lab., Dept. of Cell and Computational Biology, University of California, Davis The Vale Lab., Dept. of Cellular and Molecular Pharmacology, University of California, San Francisco The Wadsworth Lab., Biology Dept., University of Massachusetts, Amherst The Kiehart Lab., Dept. of Biology, Duke University HSC Core Facilities School of Medicine, University of Utah Indiana Center for Biological Microscopy, Indiana University Medical Center Ehlers Laboratory - Department of Neurobiology, Duke University Bob Goldstein Lab., University of North Carolina Chapel Hill Andrew Matus Lab., at Friedrich Miescher Institute for Biomedical Research Laboratory of Developmental Dynamics,Graduate School of Life Sciences, Tohoku University Zena Werb Lab., Anatomy, University of California San Francisco Satoshi Nishimura Lab., Dept. of Cardiovascular Medicine, the University of Tokyo Oshima Lab., Graduate School of Interdisciplinary Information Studies，The Univ. of Tokyo The Huser research group at UC Davis Nakano Lab., Graduate School of Science, University of Tokyo Useful Sites1) Microscopy & Imaging Resources on the WWWComplete list of all aspects of microscopy and imaging, by Douglas W. Cromey From Cellular Imaging Core of Southwest Environmental Health Sciences Center, University of Arizona College of Pharmacy, University of Arizona2) The Centro de Biologia Molecular “Severo Ochoa” (CBMSO)Contains links to general information, microscopy laboratories, publications, courses and meetings, societies, images. Especially useful for finding microscopy workshops.3) The Cell Imaging Facility, a part of the University of Utah Health Science Center's Core Research Facilities DepartmentGood explanation of CSU-Disk scanning confocal system by Chris Rodesch4)

Top of pageWhat we do in the LabWe have a lot of focus on imaging at different length scales - millimeter scale for motility tracking; microscopic regime for structure and property analysis. So, a typical day in the lab involves a lot of imaging either at the optical or fluorescence microscope or imaging using one of our semi-micro-imaging setups involving either a Precision Zoom Lens or a DSLR Macro Lens. Our Precision Zoom lens is also capable of fluorescence imaging with the help of a few band pass filters and a light source. The subjects in our experiments are microscopic compartments/droplets and we tend to program their behaviour by engineering stimuli-responsive functions and produce the stimuli locally within the microscopic compartments. So, then it becomes important to track chemical concentrations in time and across the sample space. This leads us to be interested in Spatiotemporal (space and time) analysis of chemical concentrations - which we do qualitatively using fluorescence microscope or quantitatively using a fiberoptic spectrometer. Interfacial tension plays a very important role at microscopic length scales and we like to measure it in different ways based on the system under investigation - either by using a drop shape analyzer which can by using various models infer the surface/interfacial tension and surface free energy; or by using microparticles as rheological probes to undertand viscosity of our samples.Lab momentsMoments to cherish as a labbottom of page